Age related changes in human articular chondrocyte yield, proliferation and post-expansion chondrogenic capacity
نویسندگان
چکیده
منابع مشابه
Neutral metalloproteases and age related changes in human articular cartilage.
A decrease in proteoglycan (Pg) content and disturbances in the collagen network have been reported in aging cartilage. This study aims to determine whether these changes are associated with proteolytic enzymes such as neutral metalloproteases. Eighty lateral tibial plateaus were collected from subjects after death. The age, topographical area, and lesion severity (macroscopic grading) of each ...
متن کاملAge-related changes in protein-related epitopes of human articular-cartilage proteoglycans.
Monoclonal antibodies were prepared that recognize different age-related epitopes on proteoglycan subunits of high buoyant density isolated from human epiphysial and articular cartilages. Antibody EFG-4 (IgG1) recognizes a proteinase-sensitive segment associated with the core protein. Antibody BCD-4 (IgG1) reacts with keratan sulphate bound to core protein. Both epitopes are minimally expressed...
متن کاملChondrocyte ultrastructure in adult human articular cartilage.
Age changes in the ultrastructure of articular chondrocytes have been studied in detail in mice (Silberberg, Silberberg, and Feir, 1964) and in rabbits (Davies, Barnett, Cochrane, and Palfrey, 1962; Barnett, Cochrane, and Palfrey, 1963). Less has been published concerning the influence of age on cellular ultrastructure in human articular cartilage. This is due partly to the scarcity of suitable...
متن کاملHyaluronic acid in human articular cartilage. Age-related changes in content and size.
Total tissue content and molecular mass of hyaluronic acid was determined in papain digests of human articular cartilage using a sensitive radiosorbent assay [Laurent & Tengblad (1980) Anal. Biochem. 109, 386-394]. 1) Hyaluronic acid content increased from 0.5 microgram/mg wet wt. to 2.5 micrograms/mg wet wt. between the ages of 2.5 years and 86 years. 2) Hyaluronic acid chain size decreased fr...
متن کاملAge-related changes in the composition and structure of human articular-cartilage proteoglycans.
1. Analysis of the purified proteoglycans extracted from normal human articular cartilage with 4M-guanidinium chloride showed that there was an age-related increase in their content of protein and keratan sulphate. 2. The hydrodynamic size of the dissociated proteoglycans also decreased with advancing age, but there was little change in the proportion that could aggregate. 3. Results suggested ...
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ژورنال
عنوان ژورنال: Osteoarthritis and Cartilage
سال: 2004
ISSN: 1063-4584
DOI: 10.1016/j.joca.2004.02.010